MetalloBufferTM pZn 11-7 Calibration buffer set
Data:
Introduction:
To calibrate the response of sensors such as fluorescent chemosensors against low concentrations of free (mobile, exchangeable) zinc ion near physiological pH is difficult, because of contaminating metal ions, the propensity of zinc ions to bind to ligands or glass surfaces, and the tendency of zinc ions to hydrolyse and precipitate near neutral pH. MetalloBuffers minimize these issues by effectively buffering the free zinc ion levels in a manner analogous to pH buffers. MetalloBuffer pZn levels are corrected for ionic strength and temperature effects.
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Specifications:
Product Type: Calibration buffer set for zinc sensors
Name: MetalloBufferTM pZn 11-7 Calibration buffer set
Set of10mM HEPES 10 mM Bicine 125 mM NaCl pH 7.5 2X buffers that when mixed together as directed provide fixed, low free zinc concentrations between10 pM (pZn = 11) and 0.1 μM (pZn = 7) at 25 °C.
Description:
Molecular Weight : N/A
Format: Set of bottles containing differing total zinc concentrations
Purity: As specified
Tested Application: Calibration of fluorescent zinc sensors
Solubility: Aqueous solutions
Storage: Ambient
Shipped: Ambient
Provider:
Pokegama Technologies, Inc. (KeraFast Partner)
References:
R.A. Bozym, T. Hurst, N. Westerberg, A.V. Stoddard, C.A. Fierke, C.J. Frederickson, R.B. Thompson, “Determination of zinc using carbonic anhydrase-based fluorescence biosensors,” in Methods in Enzymology: Fluorescence Spectroscopy Vol. 450 (L. Brand and M.L. Johnson, editors) New York: Elsevier, pp 279-301 (2008). PMID 19152866.
W. Stumm and J.J. Morgan, Aquatic Chemistry, 3rd Edition (Wiley-Interscience) 1996