MetalloBufferTM pZn 11-7 Calibration buffer set
MetalloBufferTM pZn 9-13 Calibration buffer set
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MetalloBufferTM pZn 11-7 Calibration buffer set
Data:
Introduction:
To calibrate the response of sensors such as fluorescent chemosensors against low concentrations of free (mobile, exchangeable) zinc ion near physiological pH is difficult, because of contaminating metal ions, the propensity of zinc ions to bind to ligands or glass surfaces, and the tendency of zinc ions to hydrolyse and precipitate near neutral pH. MetalloBuffers minimize these issues by effectively buffering the free zinc ion levels in a manner analogous to pH buffers. MetalloBuffer pZn levels are corrected for ionic strength and temperature effects.
Package Slip: Download here
Pokegama Technologies products are available through Kerafast (click on the Purchase button below); for significantly larger quantities, please contact Pokegama directly.
Specifications:
Product Type: Calibration buffer set for zinc sensors
Name: MetalloBufferTM pZn 11-7 Calibration buffer set
Set of10mM HEPES 10 mM Bicine 125 mM NaCl pH 7.5 2X buffers that when mixed together as directed provide fixed, low free zinc concentrations between10 pM (pZn = 11) and 0.1 μM (pZn = 7) at 25 °C.
Description:
Molecular Weight : N/A
Format: Set of bottles containing differing total zinc concentrations
Purity: As specified
Tested Application: Calibration of fluorescent zinc sensors
Solubility: Aqueous solutions
Storage: Ambient
Shipped: Ambient
Provider:
Pokegama Technologies, Inc. (KeraFast Partner)
References:
R.A. Bozym, T. Hurst, N. Westerberg, A.V. Stoddard, C.A. Fierke, C.J. Frederickson, R.B. Thompson, “Determination of zinc using carbonic anhydrase-based fluorescence biosensors,” in Methods in Enzymology: Fluorescence Spectroscopy Vol. 450 (L. Brand and M.L. Johnson, editors) New York: Elsevier, pp 279-301 (2008). PMID 19152866.
W. Stumm and J.J. Morgan, Aquatic Chemistry, 3rd Edition (Wiley-Interscience) 1996
MetalloBufferTM pZn 9-13 Calibration buffer set
Data:
Shown is a ratiometric calibration curve of a fluorescent-labeled apocarbonic anhydrase II with excitation at 365 and 546 nm, and emission at 617 nm.
Introduction:
To calibrate the response of sensors such as fluorescent chemosensors against low concentrations of free (mobile, exchangeable) zinc ion near physiological pH is difficult, because of contaminating metal ions, the propensity of zinc ions to bind to ligands or glass surfaces, and the tendency of zinc ions to hydrolyse and precipitate near neutral pH. MetalloBuffers minimize these issues by effectively buffering the free zinc ion levels in a manner analogous to pH buffers. MetalloBuffer pZn levels are corrected for ionic strength and temperature effects.
Package Slip: Download here
Pokegama Technologies products are available through Kerafast (click on the Purchase button below); for significantly larger quantities, please contact Pokegama directly.
Specifications
Product Type: Calibration buffer set for zinc sensors
Name: MetalloBufferTM pZn13-9 Calibration buffer
Set of 2x 10mM HEPES 10 mM NTA 125 mM NaCl pH 7.5 buffers that when mixed together as directed provide fixed, low free zinc concentrations between 0.1 pM (pZn = 13) and 1.0 nM (pZn = 9) at 25 ° C..Description:
Description:
Molecular Weight : N/A
Format: Set of bottles containing differing total zinc concentrations
Purity: As specified
Tested Application: Calibration of fluorescent zinc sensors
Solubility: Aqueous solutions
Storage: Ambient
Shipped: Ambient
Provider:
Pokegama Technologies, Inc. (KeraFast Partner)
Comments:
Buffers are provided as 2x solutions: e.g., to get final concentrations of buffer, buffer is mixed 1:1 v/v with distilled water. See enclosed instructions, also on our web site: www.Pokegamatech.com.
References:
R.A. Bozym, T. Hurst, N. Westerberg, A.V. Stoddard, C.A. Fierke, C.J. Frederickson, R.B. Thompson, “Determination of zinc using carbonic anhydrase-based fluorescence biosensors,” in Methods in Enzymology: Fluorescence Spectroscopy Vol. 450 (L. Brand and M.L. Johnson, editors) New York: Elsevier, pp 279-301 (2008). PMID 19152866.
W. Stumm and J.J. Morgan, Aquatic Chemistry, 3rd Edition (Wiley-Interscience) 1996